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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Cancer Res. 2014 Jul 9;12(11):1663–1676. doi: 10.1158/1541-7786.MCR-14-0136

Figure 6. Cyclosporin A induced reactive oxygen species upregulates MAPK signaling.

Figure 6

A, western blot of phospho- and total ERK and AKT in LuEC after treatment with CsA or vehicle for 5 min. Each lane is an individual sample. B & C, western blot of phospho- and total ERK in LuEC after treatment with CsA for 5 min with pre-treatment with NAC for 30 min followed by CsA or vehicle for 5 min (B), or with CsA or vehicle for the indicated time (C). A-C, phosphorylated proteins are normalized to their respective total protein levels with relative expression levels quantified on the right. Results are representative of one experiment (C, 2 hr–24 hr) or pooled from 3–5 experiments (A, B, C, 5 min–45 min). Data are shown as mean ± SD, statistical analysis was performed by a student t-test, * P<0.05.