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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Cancer Res. 2014 Jul 16;12(11):1644–1654. doi: 10.1158/1541-7786.MCR-14-0128-T

Figure 2. Absence of GPER does not affect early tumor development.

Figure 2

A) Whole mounts of number 4 abdominal mammary glands from 7-week-old WT/PyMT (n = 9) and KO/PyMT (n = 6) mice were stained with carmine to visualize hyperplasia. Using Image J software, a grid was overlaid on the image, and each box was analyzed for the presence of hyperplasia and normal epithelium. Hyperplasia was analyzed in the total gland and distal to the lymph node (dashed circle). B) Quantification of total gland hyperplasia. The number of boxes containing hyperplasia was normalized to the number of boxes containing total epithelium, which is defined as hyperplastic and normal epithelium. C) Quantification of hyperplasia distal to the lymph node performed in the same manner as in B. D) Number 2/3 mammary glands from 7-week-old WT/PyMT (n = 6) and KO/PyMT (n = 3) mice were stained with anti-phospho histone-H3 (P-H3) antibody to determine proliferation rate. Statistical analysis was done using two-tailed Student’s t-test with a p-value threshold of 0.05.