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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: JPEN J Parenter Enteral Nutr. 2014 May 16;39(6):688–697. doi: 10.1177/0148607114535265

Figure.5. Relative density of lysozyme in ileum tissue.

Figure.5

A. PN significantly decreased the band density for lysozyme in the ileum tissue compared with chow. Glutamine supplemented PN significantly increased lysozyme density in the ileum tissue compared with PN. Data are presented as mean ± SEM. #p < 0.0001 versus chow. *p < 0.0001 versus PN. B. Representative immunohistochemistry for lysozyme in ileum tissue from chow (left panel) versus PN (middle panel) showed a reduced expression of lysozyme in Paneth cells from PN mice. The addition of glutamine to PN (right panel) resulted in significant increased lysozyme expression in Paneth cells compared with PN alone (middle panel) (original magnification, ×20). Inserts: Magnifications of single crypts from the three groups (original magnification, ×40). The histological features are representative for all tissue samples studied.