. 2014 Sep 30;307(10):E928–E934. doi: 10.1152/ajpendo.00420.2014

Table 1.

List of primers for qRT-PCR used in this study

Gene	Accesion No.	Sequence	Position	Product Size, bp
β-Actin	NM_007393.3	`CTGGGACGACATGGAGAAGA`	313	205
β-Actin	NM_007393.3	`ACCAGAGGCATACAGGGACA`	517	205
GAPDH	NM_008084.2	`ATGGCCTTCCGTGTTCCTAC`	735	104
GAPDH	NM_008084.2	`GCCTGCTTCACCACCTTCTT`	838	104
HPRT	NM_013556	`CAGTCAACGGGGGACATAAA`	471	183
HPRT	NM_013556	`AGAGGTCCTTTTCACCAGCAA`	653	183
Cyclophilin A	NM_008907.1	`TGGTCTTTGGGAAGGTGAAAG`	421	109
Cyclophilin A	NM_008907.1	`TGTCCACAGTCGGAAATGGT`	529	109
Pck1	NM_011044.2	`TGGGGTGTTTGTAGGAGCAG`	1496	128
Pck1	NM_011044.2	`CCAGGTATTTGCCGAAGTTG`	1623	128
SREBP1c	XM_006532716.1	`GGAGCCATGGATTGCACATT`	107	70
SREBP1c	XM_006532716.1	`GGCCCGGGAAGTCACTGT`	176	70
AAC	NM_133360.2	`ATCCTGCGAACCTGGATTCT`	6022	158
AAC	NM_133360.2	`CCCACCAGAGAAACCTCTCC`	6179	158
FASN	NM_007988.3	`TGAGCACACTGCTGGTGAAC`	7162	200
FASN	NM_007988.3	`CAGGTTCGGAATGCTATCCA`	7361	200
SCD-1	NM_009127.4	`ATCGCCCCTACGACAAGAAC`	1106	137
SCD-1	NM_009127.4	`GTTGATGTGCCAGCGGTACT`	1242	137

qRT-PCR, quantitative real-time RT-PCR; HPRT, hypoxanthine guanine phosphoribosyl transferase; Pck1, phosphoenolpyruvate carboxykinase 1; SREBP1, sterol regulatory element binding transcription factor 1; AAC, acetyl-CoA carboxylase; FASN, fatty acyl synthase; SCD-1, stearoyl-coenzyme A desaturase 1.