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A: machinist sketch of microsystem. Thermoelectric modules are Peltier temperature control units. Measurements shown in inches. B: close up of system mounted on microscope above ×40 objective. First position (where fiber is pictured) capable of laser clamp ktr measurements. C: force, sarcomere length, and fiber length vs. time in a sample ktr experiment utilizing laser clamp (this example is a IIx fiber at 15°C and had a ktr of 24.3 s−1).
