Fig. 1.

Morphological characterization of dissociated L6-S1 dorsal root ganglion (DRG) neurons from rats injected with Fast Blue (FB) into the bladder wall. A: single DRG neurons in the same field, observed by light-field and fluorescent microscope, are shown in the top and bottom, respectively. Top: an arrow indicates a FB-positive bladder afferent neuron identified under the fluorescent light. Bottom: single DRG neurons with various green-staining intensities of isolectin B4 (IB4)-FITC. The numbers (0–2) beside the cells indicate the intensity of staining: 0, no or weak (negative) staining; 1, moderate staining; 2, intense staining. B: cell-size distributions of FB-positive and -negative DRG cells with different IB4 staining intensities (grades 0–2). Note that there is no grade 2-stained cells in FB-positive bladder afferent neurons.