Fig. 3.

Genetic inhibition of CaN decreases spontaneous local Ca²⁺ and prevents the effects of pharmacological inhibition and activation. A: original recordings exhibit local Ca²⁺ signals (sparks) in a CaN-Aα wild-type (WT) mouse compared with a CaN-Aα^−/− mouse ASMC. B: bar graphs present the mean frequency and amplitude of Ca²⁺ sparks from CaN-Aα WT and CaN-Aα^−/− mouse ASMCs. Nos. in parentheses indicate the no. of individual cells tested from a minimum of three mice. *P < 0.05 comparing CaN-Aα WT and CaN-Aα^−/−. C: effect of CAIP (20 μM) for 8 min on Ca²⁺ sparks in CaN-Aα^−/− mouse ASMCs. D: effect of nickel on the frequency and amplitude of local Ca²⁺ signaling in CaN-Aα^−/− mouse ASMCs.