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Generation of transgenic mice overexpressing Gαs. A: diagram of the DNA construct engineered for oocyte injection. SMP8, smooth muscle α-actin promoter; SV40, simian virus 40. B: PCR screen of tail-clip DNA using 1 primer from Gαs and 1 primer from the SV40 portion of the transgene (top) and expression of Gαs by RT-PCR from cultured airway smooth muscle (ASM) cells from transgenic (TG1) and nontransgenic (NTG) littermates (bottom).
