Table I.
Specific stem-loop RT and PCR primers for miR-143, miR-145 and U6
| Primer | Sequence | |
|---|---|---|
| miR-143 | ||
| RT stem-loop primer | 5'-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGAGCTACA-3' | |
| Forward primer | 5'- ATGGTTCGTGGGTGAGATGAAGCACTG-3' | |
| Reverse primer miR-145 | 5′-GTGTCGTGGAGTCGGCAATTC-3′ | |
| RT stem-loop primer | 5'-GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGGGATTC-3′ | |
| Forward primer | 5'- ATGGTTCGTGGGGTCCAGTTTTCCCAG-3′ | |
| Reverse primer | 5′-GTGTCGTGGAGTCGGCAATTC-3′ | |
| U6 | ||
| RT stem-loop primer | 5'- CGCTTCACGAATTTGCGTGTCAT -3' | |
| Forward primer | 5'- GCTTCGGCAGCACATATACTAAAAT -3' | |
| Reverse primer | 5'- CGCTTCACGAATTTGCGTGTCAT -3' | |