Table 3.

The primers, polymerase chain reaction (PCR) conditions and restricion enzymes used in this study

		Primer pairs	PCR conditions	Restriction enzymes
VDR	rs7975232 (ApaI)	5′-CAGAGCATGGACAGGGAGCAA-3′ 5′-GCAACTCCTCATGGCTGAGGTCTC-3′	95°C for 5 min (95°C for 30 s, 70°C for 30 s, 72°C for 60 s) × 35cycles 72°C for 5 min	ApaI
	rs1544410 (BsmI)	5′-CAACCAAGACTACAAGTACCGCGTCAGTGA-3′ 5′-AACCAGCGGGAAGAGGTCAAGGG-3′	95°C for 3 min (95°C for 30 s, 60°C for 30 s, 72°C for 30 s) × 35cycles 72°C for 5 min	BsmI
	rs2228570 (FokI)	5′-AGCTGGCCCTGGCACTGACTCTGCTCT-3′ 5′-ATGGAAACACCTTGCTTCTTCTCCCTC-3′	95°C for 5 min (95°C for 30 s, 61°C for 30 s, 72°C for 30 s) × 35cycles 72°C for 5 min	FokI
	rs731236 (TaqI)	5′-CAGAGCATGGACAGGGAGCAA-3′ 5′-GCAACTCCTCATGGCTGAGGTCTC-3′	95°C for 5 min (95°C for 30 s, 70°C for 30 s, 72°C for 60 s) × 35cycles 72°C for 5 min	TaqI
GC	rs7041	5′-AAATAATGAGCAAATGAAAGAAGAC-3′ 5′-CAATAACAGCAAAGAAATGAGTAGA-3′	95°C for 3 min (95°C for 30 s, 64°C for 30 s, 72°C for 30 s) × 30cycles 72°C for 7 min	HaeIII
	rs4588	5′-AAATAATGAGCAAATGAAAGAAGAC-3′ 5′-CAATAACAGCAAAGAAATGAGTAGA-3′	95°C for 3 min (95°C for 30 s, 64°C for 30 s, 72°C for 30 s) × 30cycles 72°C for 7 min	StyI
CYP2R1	rs10741657	5′-GGGAAGAGCAATGACATGGA-3′ 5′-GCCCTGGAAGACTCATTTTG-3′	95°C for 5 min (95°C for 30 s, 55°C for 30 s, 72°C for 30 s) × 35cycles 72°C for 5 min	MnlI