Fig. 5.

Maturation of interleukin (IL)-1β is inhibited in dynasore-treated group A streptococcus (GAS)-stimulated human macrophages. Macrophages were left untreated or treated with dynasore (80 μM) for 30 min prior to stimulation with live, ultraviolet (UV)-inactivated, heat-inactivated, live streptolysin O (SLO)-mutant or live streptolysin S (SLS)-mutant GAS [multiplicity of infection (MOI) 10]. Cell lysates were prepared after 24 h stimulation, proteins were separated on 15% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting was performed with rabbit anti-human IL-1β antibody. Analysis of β-actin expression functions as a loading control.