Fig. 5.

a, Flow cytometry plots of in-vitro-stimulated bronchoalveolar lavage (BAL) fluid cells of a patient with Löfgren's syndrome. Successive gating was performed on lymphocytes, CD3⁺, CD4⁺ and CD8⁺ T cells. Cytokine expression in CD4⁺ T cells in response to medium alone, purified protein derivative (PPD) and Staphylococcus enterotoxin A (SEA)/S. enterotoxin B (SEB) were evaluated by intracellular staining for detection of interferon (IFN-)γ and interleukin (IL)-17. (b) Paired analysis show frequencies of CD4⁺ and CD8⁺ T cells expressing either IFNγ, IL-17 or both cytokines simultaneously in response to superantigen (SEA/SEB) stimulation. Samples were from BAL (b; top row) and blood (b; bottom row) of sarcoidosis patients with Löfgren's syndrome (filled symbols) and sarcoidosis patients without Löfgren's syndrome (open symbols). Note different scales on axes.