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. 2014 Oct 1;178(2):373–383. doi: 10.1111/cei.12408

Fig. 4.

Fig. 4

Interleukin (IL)-17A concentrations in peripheral blood mononuclear cell (PBMC) cultures in response to the addition of recombinant human interleukin (rhIL)-27. PBMC were isolated from healthy donors and cultured in the presence of varying concentrations of rhIL-27 (0, 1, 10 or 100 ng/ml). On days 1, 3, 5 and 7 the concentration of IL-17A in the supernatant was determined by enzyme-linked immunosorbent assay (ELISA). (a) PBMC were left unstimulated; no significant differences were observed in IL-17A production (n = days 1, 8; days 3, 8; days 5, 11; days 7, 8). (b) T cells within the PBMC were stimulated by plate-bound anti-CD3 and anti-CD28. Significant differences were observed on all days (n = days 1, 8, days 3m 8, days 5, 17, days 7m 8); day 1, 0 versus 10 ng/ml P = 0·043; 0 versus 100 ng/ml P = 0·028. Day 3, 0 versus 100 ng/ml P = 0·028. Day 5, 0 versus 10 ng/ml P = 0·028; 0 versus 100 ng/ml P < 0·001. Day 7, 0 versus 10 ng/ml P = 0·043; 0 versus 100 ng/ml P = 0·017. The graph demonstrates percentage level compared to 0 ng/ml ± standard error of the mean. Wilcoxon's signed-rank test, *P < 0·05; ***P < 0·001.