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. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: J Immunol. 2014 Oct 3;193(10):5229–5239. doi: 10.4049/jimmunol.1400753

Figure 4. Fibrocytes from Vav-Col mice contain type I collagen protein even in the absence of type I collagen gene.

Figure 4

A&B) Primary lung mesenchymal cells from uninjured control (A) and Vav-Col (B) mice were FACS sorted for CD45-positive (pos) and negative (neg) populations. The Vav-Col and littermate control mice had similar proportions of CD45-pos and -neg cells. C) Immunoblot for type I collagen. CD45-neg cells have more type I collagen than CD45-pos cells. CD45-pos cells from control and Vav-Col mice have similar levels of type I collagen protein. D) PCR of DNA isolated from control and Vav-Col CD45-pos and -neg cells. Primers within the floxed region of col1a1 demonstrate absence of exons 2–5 in Vav-Col CD45-pos cells and intact col1a1 in CD45-neg cells and cells from control mice. Primers outside of the floxed region (in exon 6) were used as a loading control. E&F) CD45-pos cells from Vav-Col mice have less col1a1 exons 2–5 DNA (p<0.01) by qPCR (E) and less col1a1 mRNA by qPCR (F) (p<0.05) compared to CD45-pos cells from control mice, n=4.