Fig. 3.

Induction of intrinsic apoptosis in the transduced U2-OS cells. (a) Cells were incubated with STS (0.5 µM) for 8 h. Cell lysates were resolved by SDS-PAGE and analysed by immunoblotting against cleaved PARP-1. A representative blot of three repeats is shown. Quantitative data were obtained by integrating the intensity of the bands from the three repeats using a LI-COR system. Tub, α-tubulin. Molecular size markers are indicated on the left (kDa). (b, c) Cells were treated with STS for 8 h (b) or DOX (3 µM) for 30 h (c) and apoptosis was assessed quantitatively using Caspase-Glo. Data are shown as the mean±sd fold induction relative to EV and are representative of at least three experiments each performed in triplicate. Statistical differences between EV and anti-apoptotic protein-transduced cells were determined using an unpaired Student’s t-test (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001).