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. 2014 Apr 7;13:74. doi: 10.1186/1475-2840-13-74

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Copyright © 2014 Dou et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of OCN on the phosphorylation of Akt and eNOS in cultured HUVECs. (A) HUVECs incubated with 100 ng/mL OCN or vehicle for the indicated times. (B) The effects of OCN on Akt phosphorylation. (C) The effects of OCN on eNOS phosphorylation. (D) HUVECs incubated with the indicated concentrations of OCN or vehicle for 1 h. (E) The effects of OCN on Akt phosphorylation. (F) The effects of OCN on eNOS phosphorylation. Mean ± SEM of four independent experiments are plotted. *P < 0.05, **P < 0.01 vs. control group. OCN, osteocalcin.