Table 2.

Summary of In silico Analysis of FGFR2 Mutations

Novel FGFR2 Mutation	Predicted Effect Based on Molecular Modeling
E160A, H213Y	Negatively affect interaction with heparan sulfate
E219K	Reduced FGF- and heparin-mediated receptor dimerization
G227E, V248D	Destabilize the D2 domain
R251Q	Abrogate ligand binding
G271E	Destabilize the D3 domain
E475K	Possible increased kinase activity but less stable kinase domain
M640I, I642V	Local structural perturbations resulting in altered conformation of the activation loop and decreased kinase activity
A648T	Markedly reduced kinase activity due to misalignment of catalytic residues for phosphotransfer reaction
L770V	Impaired phosphorylation of Y769 and reduced recruitment of PLCγ