Figure 4.

The effect of Mox-LDL treatment on HUVEC cell migration. (a) HUVEC cells which migrated through membranes. HUVEC cells were incubated for 24 hours in culture medium supplemented with mock medium, native LDL, or Mox-LDL and then seeded onto the membrane in the upper chamber of a modified Boyden chamber in conditions inducing the migration to the lower chamber. After 8 hours cells that migrated to the lower membrane side were fixed and stained. The results shown are representative of three independent experiments performed in duplicate. (b) Quantification of (a). Pictures were taken of fixed and stained cells that migrated through the membrane. Cells were counted and numbers of migrated cells were expressed as fold over control untreated cells. Mean ± SEM on 3 independent experiments performed in duplicate. ^*** P < 0.001 versus control (ANOVA, Turkey's multiple comparison test).