Table 1.

List of the PCR primer sequences.

Gene Symbol	RefSeq Accession	Forward Primer (5′-3′)	Reverse Primer (5′-3′)	Product Size (bp)	Annealing Temperature (°C)
Actb*	NM_007393.3	CCAGATCATGTTTGAGACCTTCAA	CCAGAGGCATACAGGGACAGC	78	59
Dab2	NM_001008702.2	TCATTCTCCCTATGGCTTGG	GCTGAGCAGAATCCAGTTCC	83	60
Ddx3y	NM_012008.2	GGGTCTGTGATAAGGACAGTTCA	CACGACCACCAATACCATCATAG	177	60
Dhcr24	NM_053272.2	CTCTGGGTGCGAGTGAAGG	TTCCCGGACCTGTTTCTGGAT	195	60
Eif2s3x	NM_012010.3	GGTGAGGGTGGAGTGACTCT	TTCCCATGAGCTACGTGACCA	152	60
Eif2s3y	NM_012011.1	GGTGCTGTTGGAGCATTACC	TCAACTCGTCGGCTTAGAGC	269	58
Kdm5d	NM_011419.3	CCAGGATCTGACGACTTTCTACC	TTCTCCGCAATGGGTCTGATT	113	58
Kdm6a	NM_009483.1	CGGGCGGACAAAAGAAGAAC	CATAGACTTGCATCAGATCCTCC	203	60
Klk8	NM_008940.2	GATCCTGGAAGGTCGAGAGTG	CTGCTCCGGCTGATCTCTG	193	58
Midi	NM_183151.1	CTGTGACGGCACCTGTCTC	AAACGGCTGACTGTTGGTCTT	205	58
Prkdc	NM_011159.2	CTGTGACGGCACCTGTCTC	TCTCAATCTGAGGACGAATTGC	203	58
Rpl13a*	NM_009438.5	ATGAGGTCGGGTGGAAGTACC	CAGGAGTCCGTTGGTCTTGAG	179	62
Xist	NR_001570.2	GCTGGTTCGTCTATCTTGTGGG	GGATCCTGCACTGGATGAGT	225	58

^*indicates a reference/housekeeping gene.