Table 2.
Detected amino acids by HPLC of the moulted cuticle KOH hydrolysate and from decellularized animals. Amino acid analyses of fibroin (B. mori), collagen (P. americana) and resilin (S. gregaria) are included for comparison.
| amino acids |
moulted cuticle | decellularized animal | fibroin [32] B. mori |
collagen [33] P. americana |
resilin [34] S. gregaria |
|
|---|---|---|---|---|---|---|
| ASP | aspartic acid | 5.25 | 3.76 | 1.30 | 5.00 | 12.50 |
| GLU | glutamic acid | 5.49 | 3.93 | 1.00 | 9.10 | 6.20 |
| SER* | Serine | 7.79 | 17.04 | 12.10 | 3.90 | 8.00 |
| HIS | histidine | 0.14 | 0.09 | 0.20 | 0.40 | 1.70 |
| GLY* | glycine | 56.02 | 35.49 | 44.50 | 32.00 | 26.90 |
| THR | threonine | 1.01 | 3.49 | 0.90 | 2.40 | 3.70 |
| ARG | arginine | 0.46 | 0.94 | 0.50 | 4.50 | 6.50 |
| ALA | alanine | 5.82 | 15.62 | 29.30 | 7.80 | 9.45 |
| TYR* | tyrosine | 7.00 | 4.40 | 5.20 | — | 4.00 |
| MET | methionine | 0.06 | 0.33 | 0.10 | — | — |
| VAL | valine | 0.47 | 1.07 | 2.20 | 2.20 | 3.10 |
| PHE | phenylalanine | 1.77 | 2.54 | 0.60 | 1.30 | 4.40 |
| ILE | isoleucine | 0.50 | 1.07 | 0.70 | 1.60 | 1.90 |
| LEU | leucine | 5.07 | 6.77 | 0.50 | 3.50 | 2.60 |
| LYS | lysine | 0.55 | 0.27 | 0.30 | 2.00 | 0.85 |
| CYS | cysteine | — | — | 0.20 | — | — |
| PRO | proline | 1.61 | 2.00 | 0.30 | 12.00 | 8.20 |
| HYP | hydroxyproline | — | — | — | 10.70 | — |
| HYL | hydroxylysine | — | — | — | 1.60 | — |