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Fig. 6. Development of vessel elements in the elongating leaf blade. Longitudinal sections of protoxylem elements at 6 (A) and 15 (B) mm distal to the ligule. Elongation increases the distance between annular wall thickenings of protoxylem vessels. Bar = 50 µm. C, Transverse CWA per leaf of xylem vessel elements at five locations distal to the ligule. Mean metaxylem wall thickness at each location is indicated numerically on the figure; metaxylem cell wall thickness LSD0·05 = 0·90 µm (n = 3).