Table 2.
Selective amplification primers tested for AFLP analysis
| EcoRI primer anchor | Fluorescent label | MseI primer anchor | EcoRI primer anchor | Fluorescent label | MseI primer anchor |
| 01 AAC | TAMRA | CAG | 17 AAC | TAMRA | CTA |
| 02 AAC | TAMRA | CAC | 18 AAC* | TAMRA | CAT |
| 03 AAG | JOE | CAG | 19 AAG | JOE | CTC |
| 04 AAG | JOE | CAC | 20 AAG | JOE | CAT |
| 05 ACA | FAM | CAG | 21 ACA | FAM | CTA |
| 06 ACA* | FAM | CAA | 22 ACA | FAM | CAT |
| 07 ACC | TAMRA | CAG | 23 ACC | TAMRA | CTA |
| 08 ACC | TAMRA | CAC | 24 ACC* | TAMRA | CAT |
| 09 ACG | JOE | CAG | 25 ACG | JOE | CTA |
| 10 ACG | JOE | CAC | 26 ACG | JOE | CAT |
| 11 ACT | FAM | CAG | 27 ACT | FAM | CTA |
| 12 ACT† | FAM | CAA | 28 ACT | FAM | CTC |
| 13 AGC | TAMRA | CAG | 29 AGC | TAMRA | CTA |
| 14 AGC | TAMRA | CAC | 30 AGC | TAMRA | CAT |
| 15 AGG | JOE | CAG | 31 AGG | JOE | CTA |
| 16 AGG | JOE | CAC | 32 AGG | JOE | CAT |
See ABI kit for further details on primer composition.
* The most successful primers that were used to screen a further 75 Miscanthus accessions.
† A successful primer combination used in Hodkinson et al. (2002) but not in this study.