Table 1.
Wigglesworthia genes displaying significant differential expression in trypanosome stimulated vs. non-stimulated flies, 3 days post-blood meal uptake.
| Gene symbols | Expression | GenBank | Name |
|---|---|---|---|
| cspE | Down | WIGMOR_0606__cspE | DNA-binding transcriptional repressor |
| secY | Down | WIGMOR_0186__secY | Preprotein translocase membrane subunit |
| groL | Down | WIGMOR_0521__groL | Cpn60 chaperonin GroEL, large subunit of GroESL |
| Down | WIGMOR_0357 | MarR family transcriptional regulator | |
| sucC | Down | WIGMOR_0345__sucC | Succinyl-CoA synthetase, beta subunit |
| aspS | Down | WIGMOR_0121__aspS | Aspartyl-tRNA synthetase |
| ycfH | Down | WIGMOR_0102__ycfH | Putative metallodependent hydrolase |
| rpoC | Down | WIGMOR_0228__rpoC | RNA polymerase, beta prime subunit |
| motA | Down | WIGMOR_0035__motA | Proton conductor component of flagella motor |
| ybgF | Down | WIGMOR_0352__ybgF | SecB-dependent secretory protein |
| flhC | Down | WIGMOR_0034__flhC | Family flagellar transcriptional activator |
| accC | Down | WIGMOR_0138__accC | Acetyl-CoA carboxylase, biotin carboxylase subunit |
| yccA | Down | WIGMOR_0220__yccA | Inner membrane protein |
| lpxA | Down | WIGMOR_0385__lpxA | UDP-N-acetylglucosamine acyltransferase |
| grpE | Down | WIGMOR_0116__grpE | Pseudo |
| rpoZ | Down | WIGMOR_0359__rpoZ | RNA polymerase subunit omega |
| alaS | Down | WIGMOR_0548__alaS | Alanyl-tRNA synthetase |
| fusA | Down | WIGMOR_0032__fusA | GTP-binding protein chain elongation factor EF-G |
| Down | WIGMOR_0040 | Family flagellar basal-body P-ring formation protein | |
| mltC | Down | WIGMOR_0079__mltC | Membrane-bound lytic murein transglycosylase C |
| thiL | Down | WIGMOR_0293__thiL | Thiamin-monophosphate kinase |
| znuA | Down | WIGMOR_0125__znuA | Periplasmic component of a high-affinity zinc uptake System |
| mrcB | Down | WIGMOR_0148__mrcB | Penicillin-binding protein 1B (PBP1B) |
| gntY | Down | WIGMOR_0476__gntY | Putative gluconate transport associate protein |
| tmk | Down | WIGMOR_0100__tmk | Thymidylate kinase |
| secA | Down | WIGMOR_0585__secA | Preprotein translocase subunit, ATPase |
| gyrA | Down | WIGMOR_0315__gyrA | DNA gyrase subunit A |
| hemF | Down | WIGMOR_0163__hemF | Coproporphyrinogen III oxidase |
| rplP | Down | WIGMOR_0199__rplP | 50S ribosomal protein L16 |
| rpsG | Down | WIGMOR_0031__rpsG | 30S ribosomal protein S7 |
| ftsA | Down | WIGMOR_0582__ftsA | Cell division protein |
| groS | Down | WIGMOR_0520__groS | Cpn10 chaperonin GroES, small subunit of GroESL |
| def | Down | WIGMOR_0262__def | Peptide deformylase |
| Down | WIGMOR_0349 | TolA family protein | |
| putA | Down | WIGMOR_0327__putA | Transcriptional repressor/proline dehydrogenase/delta-1- pyrroline-5-carboxylate dehydrogenase |
| serS | Down | WIGMOR_0269__serS | Seryl-tRNA synthetase |
| ispG | Down | WIGMOR_0176__ispG | 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate Synthase |
| hslU | Down | WIGMOR_0502__hslU | Molecular chaperone and ATPase component of HslUV protease |
| thiE | Down | WIGMOR_0249__thiE | Thiamin phosphate synthase |
| Down | WIGMOR_0310 | ncRNA | |
| rpsR | Down | WIGMOR_0517__rpsR | 30S ribosomal protein S18 |
| sucA | Down | WIGMOR_0343__sucA | Thiamin-requiring 2-oxoglutarate decarboxylase |
| fliN | Down | WIGMOR_0056__fliN | Flagellar motor switching and energizing component |
| kdsA | Down | WIGMOR_0424__kdsA | 3-deoxy-D-manno-octulosonate 8-phosphate synthase |
| bioC | Down | WIGMOR_0304__bioC | Malonyl-CoA methyltransferase |
| fliF | Down | WIGMOR_0064__fliF | Flagellar basal-body MS-ring and collar protein |
| Down | WIGMOR_0083 | Pyrroline-5-carboxylate reductase | |
| holE | Down | WIGMOR_0570__holE | DNA polymerase III subunit theta |
| pyrI | Down | WIGMOR_0154__pyrI | Aspartate carbamoyltransferase, regulatory subunit |
| tyrS | Down | WIGMOR_0429__tyrS | Tyrosyl-tRNA synthetase |
| dapF | Down | WIGMOR_0508__dapF | Diaminopimelate epimerase |
| Down | WIGMOR_0443 | YhbN family protein | |
| dapA | Down | WIGMOR_0437__dapA | Dihydrodipicolinate synthase |
| ydjM | Down | WIGMOR_0156__ydjM | Putative inner membrane protein |
| fmt | Down | WIGMOR_0263__fmt | 10-formyltetrahydrofolate:L-methionyl- tRNA(fMet) N-formyltransferase |
| gpsA | Down | WIGMOR_0210__gpsA | Glycerol-3-phosphate dehydrogenase |
| recB | Down | WIGMOR_0512__recB | Exonuclease V subunit beta |
| pepA | Down | WIGMOR_0300__pepA | Aminopeptidase A |
| folD | Down | WIGMOR_0539__folD | Bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase |
| rpsK | Down | WIGMOR_0183__rpsK | 30S ribosomal protein S11 |
| ileS | Down | WIGMOR_0483__ileS | Isoleucyl-tRNA synthetase |
| motB | Down | WIGMOR_0036__motB | Family proton-channel complex protein |
| ycfH | Down | WIGMOR_0102__ycfH | Putative metallodependent hydrolase |
| Down | WIGMOR_0595 | GltJ family glutamate/aspartate transport system permease protein | |
| rpsI | Down | WIGMOR_0645__rpsI | 30S ribosomal protein S9 |
| rpoH | Down | WIGMOR_0076__rpoH | RNA polymerase, sigma 32 (sigma H) factor |
| Down | WIGMOR_0325 | tRNA | |
| ribB | Down | WIGMOR_0545__ribB | 3,4-dihydroxy-2-butanone-4-phosphate synthase |
| acpP | Down | WIGMOR_0097__acpP | Acyl carrier protein (ACP) |
| nadA | Down | WIGMOR_0624__nadA | Quinolinate synthase subunit A |
| pal | Up | WIGMOR_0351__pal | Peptidoglycan-associated outer membrane lipoprotein |
| Up | WIGMOR_0666 | tRNA | |
| yebA | Up | WIGMOR_0126__yebA | Putative peptidase |
| rplX | Up | WIGMOR_0195__rplX | 50S ribosomal protein L24 |
| yggV | Up | WIGMOR_0082__yggV | dITP/XTP pyrophosphatase |
| guaB | Up | WIGMOR_0672__guaB | IMP dehydrogenase |
| Up | WIGMOR_0558 | tRNA | |
| rplB | Up | WIGMOR_0203__rplB | 50S ribosomal protein L2 |
| hflC | Up | WIGMOR_0597__hflC | Modulator for HflB protease specific for phage lambda cII repressor |
| rnhA | Up | WIGMOR_0072__rnhA | Ribonuclease HI |
| Up | WIGMOR_0662 | tRNA | |
| sucD | Up | WIGMOR_0346__sucD | Succinyl-CoA synthetase subunit alpha |
| purD | Up | WIGMOR_0245__purD | Phosphoribosylglycinamide synthetase phosphoribosylamine-glycine ligase |
| metG | Up | WIGMOR_0214__metG | Methionyl-tRNA synthetase |
| rpsC | Up | WIGMOR_0200__rpsC | 30S ribosomal protein S3 |
| flgE | Up | WIGMOR_0044__flgE | Flagellar hook protein |
| zapA | Up | WIGMOR_0328__zapA | Protein that localizes to the cytokinetic ring |
| fumC | Up | WIGMOR_0426__fumC | Fumarate hydratase |
| Up | WIGMOR_0128 | tRNA | |
| Up | WIGMOR_0135 | tRNA | |
| metK | Up | WIGMOR_0454__metK | Methionine adenosyltransferase 1 |
| infC | Up | WIGMOR_0086__infC | Protein chain initiation factor IF-3 |
| sucB | Up | WIGMOR_0344__sucB | Dihydrolipoyltranssuccinase |
| ftsH | Up | WIGMOR_0553__ftsH | Subunit of integral membrane ATP-dependent zinc metallopeptidase |
| Up | WIGMOR_0134 | 16S ribosomal RNA | |
| htpX | Up | WIGMOR_0111__htpX | Putative endopeptidase |
| Up | WIGMOR_0237 | tRNA | |
| rnhA | Up | WIGMOR_0072__rnhA | Ribonuclease HI |
| mreB | Up | WIGMOR_0141__mreB | Family actin-like cell wall component |
| ksgA | Up | WIGMOR_0022__ksgA | S-adenosylmethionine-6-N′,N′-adenosyl (rRNA) dimethyltransferase |
Wigglesworthia genes with significant differential expression, p < 0.05.