Table 2.
Serum concentrations of cytokines from untreated, DMBA only and DMBA + ROSI-treated strains
| PPARγ-WT | PPARγ-MSE KO | |||||
|---|---|---|---|---|---|---|
| Untreated (n = 6) | DMBA only (n = 4) | DMBA + ROSI (n = 4) | Untreated (n = 6) | DMBA only (n = 4) | DMBA + ROSI (n = 4) | |
| Cytokine [signif] | mean ± SD; all values expressed as pg/ml | |||||
| IL-1α [t] | 237.6 ± 218.8 | 50.9 ± 39.5 | 31.3 ± 21.3 | 98.4 ± 78.5 | 56.5 ± 79.1 | 25.5 ± 26.5 |
| IL-1β | 185.2 ± 45.4 | 349.1 ± 213.0 | 258.3 ± 44.1 | 230.5 ± 27.6 | 221.5 ± 37.1 | 208.3 ± 99.0 |
| IL-2 | 65.4 ± 74.1 | 33.0 ± 39.4 | 14.9 ± 17.4 | 20.9 ± 11.0 | 14.7 ± 15.0 | 32.1 ± 12.4 |
| IL-3 | 2.7 ± 6.6 | ND | 9.0 ± 17.9 | 5.4 ± 1.8 | 3.5 ± 5.0 | 29.9 ± 49.6 |
| IL-4 [t] | 10.4 ± 1.1 | 13.0 ± 2.9 | 10.7 ± 1.5 | 10.7 ± 1.9 | 12.1 ± 1.5 | 11.3 ± 0.6 |
| IL-5 [g] | 13.6 ± 12.1 | 9.3 ± 13.5 | 32.2 ± 27.9 | 3.4 ± 6.0 | ND | 12.3 ± 18.4 |
| IL-6 [t] | 4.9 ± 2.3 | 25.5 ± 26.0 | 13.4 ± 9.7 | 5.9 ± 1.1 | 22.0 ± 11.2 | 60.4 ± 54.6 # |
| IL-9 | ND | ND | ND | ND | ND | ND |
| IL-10 | 29.8 ± 23.7 | 109.1 ± 131.1 | 83.0 ± 54.2 | 9.3 ± 18.8 | 35.3 ± 20.2 | 66.4 ± 7.3 |
| IL-12(p40) [i,t,g] | 502.7 ± 97.6 | 1482.0 ± 910.8 | 1804.0 ± 958.1* | 694.6 ± 402.5 | 1001.0 ± 284.3 | 728.2 ± 215.8 |
| IL-12(p70) | ND | ND | ND | ND | ND | ND |
| IL-13 | 95.8 ± 88.0 | 157.5 ± 115.4 | 125.4 ± 99.0 | 80.2 ± 52.2 | 75.5 ± 29.4 | 86.5 ± 54.4 |
| IL-17A | 3.5 ± 8.6 | 12.2 ± 14.4 | 11.89 ± 13.84 | 4.7 ± 6.8 | 6.6 ± 2.9 | 12.9 ± 12.5 |
| Eotaxin [g] | 174.0 ± 271.3 | 379.3 ± 414.7 | 417.8 ± 835.7 | 406.8 ± 116.1 | 1207.0 ± 872.2 | 979.2 ± 815.2 |
| G-CSF [t] | 153.5 ± 105.6 | 2901.0 ± 4540.0 | 2269.0 ± 1963.0 | 106.0 ± 51.1 | 3071.0 ± 2484.0 | 1706.0 ± 1310.0 |
| GM-CSF | 94.4 ± 135.1 | 241.7 ± 153.2 | 126.3 ± 51.0 | 187.9 ± 73.8 | 174.8 ± 40.0 | 164.6 ± 32.4 |
| IFN-γ | 11.2 ± 9.4 | 15.3 ± 12.2 | 6.3 ± 1.9 | 10.7 ± 5.1 | 16.5 ± 5.4 | 17.2 ± 5.7 |
| KC [t] | 10.7 ± 4.3 | 27.5 ± 27.6 | 10.0 ± 8.9 | 7.0 ± 2.4 | 26.5 ± 13.5 | 17.1 ± 7.5 |
| MCP-1 | 382.2 ± 822.0 | 541.0 ± 847.1 | 138.5 ± 69.4 | 80.0 ± 40.0 | 173.7 ± 103.1 | 698.3 ± 1192.0 |
| MIP-1α [t] | 32.8 ± 20.1 | 65.7 ± 50.1 | 36.3 ± 15.1 | 34.0 ± 2.8 | 53.4 ± 9.3 | 66.0 ± 8.7 |
| MIP-1β [t] | 12.9 ± 9.3 | 33.0 ± 25.9 | 19.9 ± 6.0 | 19.4 ± 8.0 | 47.9 ± 40.7 | 17.5 ± 6.3 |
| RANTES [ggg] | 24.7 ± 16.4 | 23.6 ± 13.2 | 15.8 ± 10.0 | 51.8 ± 6.2* | 29.8 ± 14.4 | 39.7 ± 14.0 |
| TNF-α | 191.3 ± 149.4 | 448.5 ± 247.3 | 237.9 ± 80.0 | 203.7 ± 67.2 | 243.3 ± 81.3 | 379.4 ± 206.3 |
| PGE metabolites | 425.6 ± 235.7 (n = 5) | 1953.3 ± 3544.6 (n = 4) | 278.3 ± 102.0 (n = 4) | 713.0 ± 889.1 (n = 4) | 1350.7 ± 1865.2 (n = 4) | 484.3 ± 447.6 (n = 4) |
Concentrations reported as mean ± standard deviation (SD) and expressed as pg/ml. Except for PGE metabolites, which were analyzed with a separate ELISA kit, all cytokine concentrations were obtained by a multiplex array. *, Significantly different as compared to untreated PPARγ-WT, p < 0.05; #, significantly different compared to Untreated PPARγ-MSE KO, p < 0.05; i, interaction different, p < 0.05; t, treatment different, p < 0.05; g, genotype different, p < 0.05; ggg, genotype different, p < 0.001.