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. 2014 Apr 2;14:86. doi: 10.1186/1471-2229-14-86

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Copyright © 2014 Nishihara et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Transient expression assay for activation ability of F3H promoters in Arabidopsis suspension cells. Suspension-cultured cells of Arabidopsis thaliana (T87 line) were used for this analysis. GtMYB3 and GtbHLH1 genes that can activate late-stage anthocyanin biosynthetic genes were co-introduced with various F3H promoters via PEG-mediated transformation. After 24 h culture, firefly luciferase (FLUC) activity was measured with a luminometer. A Renilla luciferase (RLUC)-driven Cauliflower mosaic virus (CaMV) 35S promoter was used for internal standardization of PEG infection. Relative activities (LUC/RLUC) are shown. Asterisks (**) indicate significant differences between TfF3H pro (1 kbp) and other constructs (P < 0.01, t-test).