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. 2014 Apr 7;13:50. doi: 10.1186/1475-2859-13-50

Table 2.

Plasmids and oligonucleotides used

Plasmid, or primer Genotype or sequence Reference or source
Plasmid
  
  
pAB49
 Same as pGFPmut3.1 plus Δplac plus psoxS
 This study
pAB43
 Same as pGFPmut3.1 plus Δplac
 This study
pAB828
 Same as pGFPmut3.1 plus ::lacZ-GFPmut3.1 fusion
 This study
Primers
  
  
GFPmut-A
 ATGACCATGATTACGCCAAGCTAGTAGGCCTGATAGTAGACTCTAGAGGATCCCCGGGTA
  
GFPmut-B
 TACCCGGGGATCCTCTAGAGTCTACTATCAGGCCTACTAGCTTGGCGTAATCATGGTCAT
  
soxS-GFP-A
 TTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGTAAATCGCTTTACCTCAAGTTAACTTGAGGAATTATACTCCCCAACAGATGAATTAACGAACTGAACACTGAAAAGAGGCAGATTTATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTG
  
soxS-GFP-B CACCCTCTCCACTGACAGAAAATTTGTGCCCATTAACATCACCATCTAATTCAACAAGAATTGGGACAACTCCAGTGAAAAGTTCTTCTCCTTTACTCATAAATCTGCCTCTTTTCAGTGTTCAGTTCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTACTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAA