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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Mol Immunol. 2013 Dec 3;58(1):56–65. doi: 10.1016/j.molimm.2013.10.013

Fig. 2.

Fig. 2

Live cell images depict antigen transfer from B cells to dendritic cells. BJAB cells were labeled with CTO and pulsed with antigen. Primary myeloid DCs (a) or monocyte derived DCs (b) were labeled with CTFR and then co-cultured with either aIg (upper panels) or non-specific Ab (lower panels) pulsed B cells in glass bottom dishes. After 18 hr, live cell images were taken by confocal microscopy (600X, 2x zoom; arrows point to DCs that bear antigen).