Skip to main content
. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Mol Immunol. 2013 Dec 3;58(1):56–65. doi: 10.1016/j.molimm.2013.10.013

Fig. 6.

Fig. 6

Primary B cells transfer antigen to dendritic cells by an SR-A mediated mechanism. Primary DCs were either left untreated (UT) or treated with aSR-A Ab for 1 hr prior to being co-cultured with primary B cells pulsed with the following: no Ag (gray curve) non-specific Ab (dotted curve) or aIg (black curve). Cells were harvested and stained for flow cytometry. (a) Primary B cells are capable of transferring antigen. Antigen levels (AF488) were assessed for CD19−:CD14−:CD11c+ gated population for untreated DC co-cultures. (b) Anti-SR-A Ab significantly inhibits antigen transfer from primary B cells. MFI values for transferred antigen (AF488) were determined for DCs either left untreated or treated with aSR-A Ab from 3 independent experiments (* p<0.001; n=3; thin bars represent value range). (c) Primary B cells (gated by CD19) were assayed for degree of apoptosis by TO-PRO-3. Negative (untreated) primary B cells, aSR-A treated primary B cells, and B cells triggered for apoptosis by incubation at 65°C for 30 min. are illustrated.