Skip to main content
. Author manuscript; available in PMC: 2015 Nov 15.
Published in final edited form as: J Immunol. 2014 Oct 17;193(10):4971–4979. doi: 10.4049/jimmunol.1401264

Figure 4. Antigen-induced PCs colonize the B220+ BM PC subset for months post-immunization.

Figure 4

(A) Adult B6 mice were immunized with NP-CγG, and at the indicated time points B220+ and B220− BM PCs were sorted into NP26-BSA coated ELISPOT plates that were subsequently probed with anti-Igκ and Igλ antibodies. Numbers of hapten-specific PCs in the BM at each time point were calculated as described in Methods. Error bars represent SEM of triplicate ELISPOT wells. Background has been subtracted out. *, p<0.05. (B) B6 mice were immunized with NP-CγG. At d30 post-immunization mice were fed BrdU (or not) for an additional 7 days, and BM CD138high B220+/− PCs evaluated for intracellular NP-binding and BrdU incorporation. 8 × 106 events were collected per file.