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. Author manuscript; available in PMC: 2014 Nov 17.
Published in final edited form as: Chem Res Toxicol. 2013 Jun 20;26(7):1034–1042. doi: 10.1021/tx400078b

Figure 1. Differential Sensitivity of Hearts and Lung Transcriptomes to MS and SS Cigarette Smoke and High Fat Diet-Induced Obesity.

Figure 1

Panel A: The number of statistically significant (p<0.01) genes differentially expressed in lungs (hashed bars) or hearts (solid bars) of RW or DIO C57BL/6 mice exposed to repeated MS or SS smoke exposures as described in Experimental Procedures. Gene changes were assessed from whole genome microarray data of RNA extracted from whole tissues as described. Panels B & C: Principal Component Analysis of All 3,012 Pulmonary (B) and 3,252 Cardiac (C) Genes by treatment using normalized intensity values. Each point represents an individual animal (N=8 per group) from each treatment group using the following colored symbols: RW-SC (green), RW-MS (gray), RW-SS (magenta), DIO-SC (dark blue), DIO-MS (red), and DIO-SS (brown). Principal components analysis was performed on treatment groups with GeneSpring v.11 (Silicon Genetics) using non-transformed (ratio) normalized intensity values. Variances are described for the lung data (panel B) such that principal component 1 represents the variance on the effect of exposure and principal component 2 describes the variance due to the effect of diet phenotype (obesity). For the heart data (panel C), principal component 1 and 2 represent the variance on the effect of diet phenotype (obesity) and exposure, respectively.