Table 1.
Transcriptional Responses of the RW Heart to MS- and SS-Smoke: Most Sensitive Genesa
| Fold-Change | ||||
|---|---|---|---|---|
| Gene Symbol | Gene Name | MS | SS | DIO Regb |
| Energy Metabolism | ||||
| Pdk4 | Pyruvate dehydrogenase kinase, 4 | ---- | 1.7 | Y |
| Glu1 | Glutamine synthetase | 1.4 | 1.6 | N |
| Iron homeostasis | ||||
| Slc25a37 | Mitoferrin-1 | 1.5 | 2.1 | N |
| Tfrc | Transferrin receptor | −3.4 | −4.1 | N |
| Cp | Ceruloplasmin/ferroxidase | −1.4 | −1.8 | N/Y |
| Alas1 | Aminolevulinic acid synthase 1 | −1.4 | −1.7 | N |
| Stress/Inflammation | ||||
| Ddit4 | DNA damage inducible transcript 4 (RTP301) | 1.3 | 2.1 | N |
| Mkrn1 | Makorin, ring finger protein, 1 | ---- | 1.7 | N |
| Rit1 | Ras-like, without CAAX 1, GTPase | 1.4 | 1.7 | N |
| Cebpb | CCAAT/enhancer binding protein β, transcription factor | 1.4 | 1.7 | N |
| C es3 | Carboxylesterase 3 | 1.3 | 1.5 | N |
| Nr1d1 | Rev-Erb A alpha transcription factor | −1.9 | −2.2 | N/Y |
| Nr3c1 | Glucocorticoid receptor transcription factor | ---- | −2.1 | N |
| Dbp | D site albumin promoter binding protein | −1.3 | −2.0 | N |
| Tnfsf12a | TNF receptor, member 12 (TWEAKR/Fn14) | −1.7 | −1.7 | N |
| Dct | Dopachrome tautomerase | −1.5 | −1.4 | N |
| Proliferation/cell cycle | ||||
| Bptf | Bromodomain PHD finger transcription factor | ---- | −1.9 | N |
| Ccnd1 | Cyclin D1 | −1.3 | −1.8 | N |
| Bhlhe40 | Basic helix-loop-helix family, e40 (DEC1) | −1.3 | −1.7 | N/Y |
| Sfrs3 | Splicing factor, Arg/Ser-rich 3 (SRp20) | −1.5 | −1.6 | N |
| Ion Transport | ||||
| Kcnd2 | Potassium voltage gated channel, K4.2 | ---- | −1.9 | N |
| Kcne1 | K voltage gated channel | −2.1 | −1.8 | Y |
| Slc41a3 | MgtE, Na-Mg exchanger | −1.6 | −1.5 | N |
| Vascular Tone | ||||
| Snca | a-Synuclein | ---- | 2.8 | N |
| Nampt | Nicotinamide phosphoribosyl transferase (Visfatin) | 1.4 | 1.6 | Y |
| Ptgds | Prostaglandin D2 synthase | −1.6 | −1.8 | Y |
| Nppb | Natriuretic peptide precursor B (BNP) | −1.7 | −1.8 | N/Y |
| Slmap | Sarcolemma associated protein | ---- | −1.8 | N |
| Cald1 | Caldesmon 1 | ---- | −1.8 | N |
| Npr3 | Natriuretic peptide receptor 3 | −1.4 | −1.6 | N |
Selected 30/108 significant (p<0.01) cardiac genes regulated >1.5-fold by either mainstream smoke (MS) or sidestream smoke (SS), relative to RW mice exposed to filtered air (controls). Alternate gene or gene product names are included. (The complete list of regulated cardiac genes is available in Supporting Table S3). The extent of regulation is indicated as fold-change with missing values (----) indicating no significant gene change. Highlighted entries indicate genes previously reported to be regulated by HIF-1α. 69–86
Y (yes) or N (no) indicates whether a gene was significantly regulated (p<0.01) in MS and SS smoke-exposed DIO mice; N/Y indicates that the gene was regulated in the DIO heart exposed to SS smoke, but not to MS smoke.