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. 2014 Apr 3;15:54. doi: 10.1186/1471-2369-15-54

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Copyright © 2014 Edwards et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immunolocalization of CLIC4 in mouse kidney, high power images. Vibratome sections stained with affinity-purified antibody to CLIC4 (red) plus markers for proximal tubule brush border (Lectin LTA, here shown in blue), endothelial cells (Lectin IB4, green), and nuclei (DAPI, cyan) were observed with a 60× objective under confocal microscopy. The upper set of images (A-C) are from a WT mouse, the lower set (D-F) are from a Clic4 null mouse. A and D (left): CLIC4 signal alone (red); B and E (center): merged image of the three markers (proximal tubule brush border in blue, endothelial cells in green, and nuclei in cyan); C and F (right): merged image of all signals. Solid arrow: colocalization of CLIC4 with LTA in proximal tubule brush border. Solid arrow head: CLIC4 signal in nucleus of a proximal tubule cell. Open arrow: colocalization of CLIC4 and IB4 in glomerular capillary loop. Open arrowhead: colocalization of CLIC4 and IB4 in peritubular capillary. Scale bar represents 50 microns.