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. 2014 Aug 7;3(5):630–641. doi: 10.1002/mbo3.194

Figure 3.

Figure 3

Arginine promotes swimming through conservation of μH+. (A–C) Motility agar plates (MOT) with or without 20 mmol/L arginine were stab inoculated with Proteus mirabilis HI4320. Disks impregnated with DMSO or 10 mmol/L CCCP were placed on the surface of the plates, and the zone of inhibition of motility was measured after an 18 h incubation at 30°C. (A) Measurement of the zone of inhibition of swimming. Error bars represent mean ± SD for four independent experiments with three replicates each. ***P < 0.001 by Student's t-test. (B) Representative image of P. mirabilis HI4320 swimming in MOT. (C) Representative image of P. mirabilis HI4320 swimming in MOT supplemented with arginine. (D) Swimming diameter was measured for P. mirabilis HI4320 and isogenic mutants after an 18-h incubation at 30°C, **P < 0.01 and ***P < 0.001 by Student's t-test compared to P. mirabilis HI4320. (E) Measurement of the zone of inhibition of swimming for P. mirabilis HI4320 and isogenic mutants in MOT agar with or without 20 mmol/L arginine. **P < 0.01 and ***P < 0.001 by Student's t-test compared to MOT.