Phosphorylation of MoHog1p MAPK in the Magnaporthe oryzae wild-type strain 70-15 and in the mutant strains ΔMohog1, ΔMohik1, ΔMosln1, and ΔMohik1/Δsln1. Mycelia of the strains M. oryzae 70-15 and the gene inactivation mutants were used for western blot analysis as described in Experimental Procedures. Incubation in CM was used as negative control (K−). The WT and the gene inactivation mutants were incubated in CM with 0.5 mol/L/1.0 mol/L NaCl or 0.5 mol/L/1.0 mol/L sorbitol. M is the biotinylated protein ladder (Cell Signaling Technology, Beverly, MA).