Figure 4.

A. Sequencing of the rRNA gene of Katablepharis CRE revealed a 332 bp region of the 28S rRNA gene that is unique to the CRE strain. This region is GC-rich compared to the rest of the gene and shows no significant similarity to other katablepharids in the NCBI database, while the rest of rRNA gene aligns well with K. japonica and other sequenced katablepharids. Colored arrows indicate PCR primers designed in the 28S variable region (purple), to be general for all eukaryotes (teal), or katablepharid-specific (light brown). Numbers within the arrows refer to the primer sets that were used to answer the research questions discussed in the introduction. B. Nucleotide sequence of the Katablepharis CRE USE (bold) with flanking LSU sequence (green). ORF primers, general eukaryotic primers used to amplify additional D2 USE, are underlined.