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. 2014 Aug 28;3(5):764–776. doi: 10.1002/mbo3.206

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© 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Comparison of Katablepharis CRE abundance estimated by FISH and qPCR using probes specific for its Unique Sequence Element (USE) within the large subunit for both approaches. The x axis corresponds to numbers of cells estimated through FISH and the y-axis to LSU copies measured by qPCR. Regression with y-intercept set to zero was used to estimate gene copy number per cell, with a slope of 2.6 and an R2 value of 0.59. Horizontal and vertical error bars indicate standard deviation for abundance estimates by FISH and qPCR, respectively.