Table 2.

Quantification of subcellular localization of GFP-VirB11 and its derivatives.

VirB11 segment	Plasmid	Total number of cells	One pole	Both poles	Others	Total number at poles	% Polar
2–343	pADI16	154	88	29	37	117	76
2–217	pADI23	364	210	62	92	272	75
149–343	pADI22	152	103	19	30	122	80
149–217	pADI30	131	67	14	50	81	62
149–248	pADI41	229	93	29	107	122	53
123–248	pADI40	209	154	23	32	177	85

Number of bacteria with one polar focus, two polar foci and other phenotypes were counted. Percent polar is defined as 100 × (number of bacteria with one and two polar foci/number of fluorescent bacteria).