Table 1.

qRT-PCR analytical conditions

Gene	Component	Concentration	Sequence 5′–3′	Accession number
Adiponectin	Forward primer	150 nM	GAGACGCAGGTGTTCTTGGT	NM_144744
	Reverse primer	200 nM	CTTCCGCTCCTGTCATTCCA
	FAM-labeled probe	100 nM	CCTAAGGGTGACCCAGGAGATGC
	MgCl2	3.5 mM
GILZ	Forward primer	150 nM	GGAGGTCCTAAAGGAGCAGATTC	NM_031345
	Reverse primer	300 nM	GCGTCTTCAGGAGGGTATTCTC
	FAM-labeled probe	100 nM	TGAGCTGGTTGAGAAGAACTCGCA
	MgCl2	3 mM
IL-6	Forward primer	250 nM	TCAACTCCATCTGCCCTTCAG	NM_012589
	Reverse primer	350 nM	TGTCAACAACATCAGTCCCAAGA
	Cy5-labeled probe	100 nM	TCTCTCCGCAAGAGACTTCCAGCCAGTT
	MgCl2	2.5 mM
TNF-α	Forward primer	200 nM	CTGTACCTTATCTACTCCCAGGTTCTC	NM_012675
	Reverse primer	200 nM	TCTCCTGGTATGAAATGGCAAA
	FAM-labeled probe	100 nM	TGCTCCTCACCCACACCGTCAGC
	MgCl2	2.5 mM

Abbreviations: Cy5, cyanine 5; GILZ, glucocorticoid-induced leucine zipper; IL, interleukin; qRT-PCR, quantitative reverse transcription polymerase chain reaction; TNF, tumor necrosis factor.