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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Prostate Cancer Prostatic Dis. 2014 Sep 23;17(4):359–365. doi: 10.1038/pcan.2014.37

Figure 1. Prostate Health Cocktail Suppresses the Proliferation of Prostate Cancer Cell Lines Dose-dependently and suppresses AR expression.

Figure 1

Figure 1

Human prostate cancer LNCaP, PC3 and LAPC3 cells were seeded into 96-well plates (3000 cells/well) to grow to 80% confluency. The dry PHC powder was dissolved in serum-free RPMI 1640 (SF-RPMI) and added to the cells at the final concentrations of 0 (SF-RPMI as the vehicle control), 0.01, 0.1 and 1 mg/ml, respectively. Following 72 hour incubation at 37°C in a 5% CO2 atmosphere, the cells were washed and subjected to the MTS assay. The absorbance was detected at 490 nm with a Microplate Reader. Optical density (OD) decreased in a dose-dependent manner, representing suppression of proliferation (A). AR expression was measured via Western Blot and also showed that PHC reduced expression in a dose-dependent pattern (B). βactin is used as control, representing protein expression of a “housekeeping” gene.