Table 1.
Effect of 6-OHDA-lesion and STN-HFS on bilateral changes of optical density measurements of immunoreactive signals for VGLUT1-3
| VGLUT1 | VGLUT2 | VGLUT3 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| |
Ipsilateral side |
Controlateral side |
Ipsilateral side |
Controlateral side |
Ipsilateral side |
Controlateral side |
||||||
|
A |
Controls |
6-OHDA |
Controls |
6-OHDA |
Controls |
6-OHDA |
Controls |
6-OHDA |
Controls |
6-OHDA |
Controls |
6-OHDA |
| CPu |
51,35 (±3,2) |
53,33 (±1,9) |
48,78 (±3,4) |
52,59(±1,7) |
20,15 (±2,38) |
12,64*(±1,5) -37% |
19,76 (±2,3) |
13,38*(±0,6) -33% |
16, 65(±0,8) |
18,15 (±1,7) |
15,37 (±0,8) |
16, 33 (±3,1) |
| PM Cx |
55,54 (±3,3) |
48,3 (±2,1) |
55,81 (±2,9) |
48,07 (±2,8) |
18,56 (±1,7) |
10,93*(±0,6) -42% |
18,5 (±2,1) |
11,12*(±0,6) -40% |
14,34 (±0,9) |
13,09 (±2,4) |
14,39 (±0,9) |
13, 02 (±2,5) |
| SS Cx |
45,93 (±3) |
35,37 (±1,6) |
45,93 (±1,6) |
38,93 (±1,6) |
15,79 (±1,81) |
9,22*(±0,4) -42% |
16,44 (±1,8) |
10,57*(±0,6) -36% |
12,01 (±0,6) |
10,28 (±2,1) |
12,89 (±0,7) |
11,63 (±2,4) |
| Acb |
54,15 (±2,8) |
63,43 (±2,8) |
51,52 (±3,6) |
59,65 (±3,1) |
22,55 (±2,7) |
13,56*(±2,1) -40% |
22,14 (±2,6) |
13,91*(±2,1) -47% |
18,84 (±0,9) |
23,33 (±1,1) |
19,04 (±1,1) |
23 (±1,2) |
| Thalamus |
41,18 (±3,4) |
34,15 (±1,7) |
41,86 (±3,3) |
32,47 (±1,1) |
16,83 (±2,3) |
8,85*(±0,4) -48% |
17,49 (±2,4) |
9,05*(±0,7) -48% |
11,38 (±0,5) |
9,85 (±1,3) |
11,43 (±0,5) |
9,79 (±1,1) |
| STN |
21,13 (±2,7) |
23, 56 (±4,6) |
19,25 (±2,5) |
20,65 (±4,2) |
16,83 (±2,1) |
7,66*(±1,3) -55% |
15,53 (±2,3) |
9,32*(±0,5) -40% |
ND |
ND |
ND |
ND |
| GP |
ND |
ND |
ND |
ND |
12,78 (±1,9) |
7,97(±1) |
13,39 (±1,7) |
8,83 (±1,1) |
ND |
ND |
ND |
ND |
| SNr |
ND |
ND |
ND |
ND |
51,1 (±2,4) |
11,26*(±0,3) -25% |
15,76 (±2,2) |
11,39*(±0,5) -28% |
ND |
ND |
ND |
ND |
| |
Ipsilateral side |
Controlateral side |
Ipsilateral side |
Controlateral side |
Ipsilateral side |
Controlateral side |
||||||
|
B |
6-OHDA |
6-OHDA + STN-HFS |
6-OHDA |
6-OHDA + STN-HFS |
6-OHDA |
6-OHDA + STN-HFS |
6-OHDA |
6-OHDA + STN-HFS |
6-OHDA |
6-OHDA + STN-HFS |
6-OHDA |
6-OHDA + STN-HFS |
| CPu |
53,33 (±1,8) |
65,07*(±3,4) +22% |
52,59 (±1,7) |
64,43(±3,2) +23% |
12,64 (±1,5) |
17,81*(±1,4) +41% |
13,38 (±0,6) |
17,61*(±1,8) +32% |
18,15 (±1,7) |
26,13*(±2,9) +44% |
16,33 (±3,1) |
24,88*(±1,8) +53% |
| PM Cx |
48,3 (±2,2) |
63,08*(±3,3) +31% |
48,07 (±2,8) |
64, 79*(±3,5) +35% |
10,93 (±0,6) |
16,93*(±1,6) +55% |
11,12 (±0,6) |
16,86*(±1,8) +52% |
13,09 (±2,4) |
20*(±1,9) +53% |
13,02 (±2,5) |
16,62*(±1,7) +51% |
| SS Cx |
35,75 (±1) |
54,25*(±3,8) +52% |
38,93 (±1,6) |
55,08*(±3,8) +41% |
9,22 (±0,4) |
14,03*(±1,4) +52% |
10,57 (±0,6) |
13,38*(±1,6) +27% |
10,28 (±2,1) |
20,44*(±2,1) +99% |
11,63 (±2,4) |
16,78*(±1,4) +44% |
| Acb |
63,43 (±2,8) |
65,39 (±3,9) |
59,65 (±4,1) |
65,06 (±4,1) |
13,56 (±2,1) |
20,25*(±1,3) +49% |
13,91 (±2,1) |
18,78*(±1,2) +35% |
23,23 (±1,1) |
31,7* (1,2) +36% |
23 (±1,2) |
31,89*(±1,2) +38% |
| Thalamus |
34,15 (±1,7) |
48,1*(±3,8) +41% |
32,47(±1,1) |
46,37 (±3,6) +43% |
8,85 (±0,4) |
14,63*(±1,6) +65% |
9,05 (±0,7) |
13,5*(±1,5) +449% |
9,85 (±1,3) |
20,3*(±1,6) +106% |
9,79 (±1,1) |
19,38*(±2,5) +98% |
| STN |
23,56 (±4,6) |
17,78 (±2,6) |
20,65 (±4,2) |
19,34 (±3) |
7,66 (±1,3) |
14,79*(±1,5) +93% |
9,32 (±0,5) |
15,94*(±1,9) +71% |
ND |
ND |
ND |
ND |
| GP |
ND |
ND |
ND |
ND |
7.97 (±1) |
10,18 (±0,9) |
8,83 (±1,1) |
9,61 (±1) |
ND |
ND |
ND |
ND |
| SNr | ND | ND | ND | ND | 11,26 (±0,3) | 15,84*(±1,3) +41% | 11,39 (±0,5) | 16,64*(±1,4) +46% | ND | ND | ND | ND |
A, Modifications of VGLUT1-3 expression induced by unilateral 6-OHDA-lesion of SNc (control rats, n = 9; 6-OHDA rats, n = 6). *p<0.05, vs control group.
B, Modifications of VGLUT1-3 expression induced by unilateral STN-HFS in 6-OHDA lesioned rats (6-OHDA rats, n = 6; 6-OHDA rats + STN-HFS, n = 10). *p<0.05, vs non stimulated 6-OHDA-lesioned group. Values of optical densities measurements are expressed as a mean ± standard error (SEM). Data were analyzed for each brain structure by Kruskal-Wallis tests with SigmaStat 3.1 software. Post-hoc analyses were carried out with the Dunn’s method. Bold numbers correspond to significant differences. Ipsilateral side: related to the lesion and/or stimulation side; Controlateral side: related to the lesion and/or stimulation side; Acb, Accumbens nucleus; CPu, Caudate Putamen (striatum); GP, Globus Pallidus; PM Cx, Premotor Cortex; SNr, Substantia nigra pars reticulata; SS Cx, Somatosensory Cortex; STN, Subthalamic nucleus; Thalamus (VL/VM).