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. 2014 Nov 17;9(11):e112619. doi: 10.1371/journal.pone.0112619

Figure 6. Didox is well tolerated.

Figure 6

A. Didox treated C57Bl/6 mice showed no difference in tissue morphology compared to vehicle treated controls as read by a veterinary pathologist blinded to treatment assignment. Representative H&E sections of gastrointestinal tract (Small Intestine) and bone marrow from Didox (n = 3) and control treated animals (n = 3). B. Colony formation assays performed on normal HSCs following 24 hour Didox exposure (0–200 µM), p = 0.09. C. Didox treatment does not harm normal HSCs. C57Bl/6 mice were treated for 5 days with 425 mg/kg Didox or a vehicle control via IP injection. 72 hours post treatment the animals were sacrificed and their marrow harvested. Marrow was then transplanted into lethally irradiated (8 Gy) Ly5.1+ recipients and allowed to engraft. Post-engraftment the animals were sacrificed, marrow harvested, and analyzed for Ly5.2+ by flow cytometry. Engraftment values were normalized to vehicle controls. N = 5 per group.