Figure 2. Specific salivary epithelial cell markers were expressed in co-cultured mMSCs, as detected by western blotting and RT-PCR analysis of cell-specific markers.

Total protein lysate and mRNA samples isolated from pSGCs from 4 week-old B6 mice were used as a positive control. mMSCs without co-culture was used as a negative control. Acinar markers of salivary specific α-AMY, M3R and AQP-5 were detected in pSGCs and co-cultured mMSCs. Densitometer analyses of the expressed proteins and genes in three independent replicates(*p<0.05, **p<0.01, one-way ANOVA with Bonferroni post-hoc test).