Table 1. Identification of proteins selected by 2DE analysis.
Spot # A) | UniProt Acc. # B) | Gene Identification | Gene abbreviation | # AA C) | M.W (kDa) D) | calc. PI E) | # Pep F) | Cov % G) |
3 | Q9R0Y5 | Isoform 2 of Adenylate kinase isoenzyme 1 | AK1 | 194 | 21.54 | 5.67 | 2 | 7.0% |
4 | P18760 | Cluster of Cofilin-1 | CFL1 | 166 | 18.50 | 8.09 | 5 | 24.0% |
5 | Q4FK36 | Destrin | DSTN | 165 | 18.50 | 8.14 | 4 | 30.0% |
6 | A2RSH1 | cAMP-specific 3′,5′-cyclic phosphodiesterase 4D | PDE4D | 754 | 86.30 | 5.07 | 3 | 8.1% |
7 | Q9R1P3 | Proteasome subunit beta type-2 | PSMB2 | 201 | 22.90 | 6.52 | 2 | 14.0% |
8 | Q9Z1R9 | MCG124046 | PRSS1 | 246 | 26.10 | 4.94 | 3 | 13.0% |
9 | Q9D967 | Magnesium-dependent phosphatase 1 | MDP1 | 164 | 18.58 | 6.29 | 1 | 8.0% |
10 | Q14AA6 | MCG49183 | Q14AA6 | 216 | 24.36 | 7.75 | 2 | 19.0% |
11 | Q69ZM6 | Isoform 2 of Serine/threonine-protein kinase 36 | STK36 | 1316 | 144.18 | 5.59 | 2 | 5.0% |
12 | P97429 | Annexin | ANXA4 | 319 | 36.00 | 5.57 | 5 | 15.0% |
13 | O08915 | AH receptor-interacting protein | AIP | 330 | 37.50 | 6.40 | 5 | 15.2% |
14 | Q9WTP6 | Isoform 2 of Adenylate kinase 2, mitochondrial | AK2 | 239 | 26.47 | 6.96 | 9 | 20.0% |
15 | Q60930 | Voltage-dependent anion-selective channel protein 2 | VDAC2 | 295 | 31.73 | 7.44 | 5 | 18.0% |
16 | Q9CPP6 | NADH dehydrogenase 1 alpha subcomplex subunit 5 | NDUFA5 | 116 | 13.36 | 7.82 | 3 | 63.0% |
17 | Q9R059 | Four and a half LIM domains protein 3 | FHL3 | 289 | 31.79 | 5.80 | 13 | 37.0% |
18 | P19157 | Glutathione S-transferase P 1 | GSTP1 | 210 | 23.60 | 7.69 | 4 | 23.0% |
19 | Q9JKX6 | ADP-sugar pyrophosphatas | NUDT5 | 218 | 23.98 | 5.34 | 5 | 18.0% |
20 | Q6ZWQ5 | Sorting nexin 12, isoform CRA_b | SNX12 | 162 | 18.90 | 8.44 | 3 | 21.0% |
21 | Q6P5G3 | MBT domain-containing protein 1 | MBTD1 | 631 | 70.67 | 7.96 | 2 | 7.0% |
22 | P26883 | Peptidyl-prolyl cis-trans isomerase FKBP1A | FKBP1A | 108 | 11.90 | 7.89 | 2 | 24.0% |
23 | P08207 | S100 calcium binding protein A10 (Calpactin), isoform | S100a10 | 97 | 11.20 | 6.77 | 9 | 48.0% |
24 | Q08024 | Isoform 2 of Core-binding factor subunit beta | CBFB | 187 | 22.03 | 5.59 | 2 | 10.0% |
25 | Q64433 | 10 kDa heat shock protein, mitochondrial | HSPE1 | 102 | 11.00 | 8.35 | 2 | 28.0% |
26 | Q6NTA4 | Ras-related GTP-binding protein B | RRAGB | 374 | 43.19 | 5.99 | 2 | 8.0% |
27 | Q564E2 | L-lactate dehydrogenase | LDHA | 332 | 36.50 | 7.61 | 2 | 10.0% |
28 | P09671 | Superoxide dismutase | SOD2 | 222 | 24.60 | 8.62 | 4 | 17.0% |
29 | Q9DAG9 | PHD finger protein 7 | PHF7 | 307 | 35.38 | 8.95 | 2 | 12.0% |
30 | Q8BZW2 | Ankyrin repeat domain-containing protein 56 | ANKRD56 | 760 | 83.54 | 8.52 | 3 | 7.0% |
31 | P62889 | Rpl30 protein (ribosomal protein L30) | RPL30 | 115 | 12.78 | 9.65 | 2 | 14.0% |
32 | P70296 | Phosphatidylethanolamine-binding protein 1 | PEBP1 | 187 | 23.00 | 5.40 | 3 | 19.0% |
33 | Q8VBV3 | Exosome complex component RRP4 | EXOSC2 | 293 | 32.63 | 7.06 | 5 | 18.0% |
34 | P63242 | Eukaryotic translation initiation factor 5A-1 | EIF5A | 154 | 16.80 | 5.24 | 5 | 21.0% |
35 | Q99LC5 | Electron transfer flavoprotein subunit alpha | ETFA | 333 | 35.00 | 8.62 | 4 | 23.0% |
36 | P68040 | Guanine nucleotide-binding protein subunit beta-2-like 1 | GNB2L1 | 317 | 35.10 | 7.69 | 2 | 12.0% |
37 | Q6ZVL3 | PDZ domain actin binding protein Shroom mRNA | Q6ZVL3 | 889 | 87.86 | 9.56 | 4 | 18.0% |
38 | Q9DBJ1 | Phosphoglycerate mutase 1 | PGAM1 | 254 | 28.83 | 6.67 | 5 | 26.0% |
39 | P50580 | Proliferation-associated protein 2G4 | PA2G4 | 394 | 43.70 | 6.41 | 3 | 19.0% |
40 | Q3UU43 | MCG16489, isoform CRA-a | CHPF2 | 768 | 85.65 | 7.64 | 2 | 7.0% |
41 | P10853 | Histone H2B (Fragment) | HIST1H2BJ | 126 | 13.94 | 10.31 | 4 | 29.0% |
42 | P17742 | Peptidyl-prolyl cis-trans isomerase A | PPIA | 164 | 18.00 | 7.90 | 2 | 16.0% |
43 | Q9Z104 | High mobility group protein 20B | HMG20B | 317 | 35.87 | 9.33 | 3 | 12.0% |
44 | O35737 | Heterogeneous nuclear ribonucleoprotein H | HNRNPH1 | 449 | 49.20 | 6.30 | 2 | 8.0% |
45 | O88712 | Isoform 2 of C-terminal-binding protein 1 | CTBP1 | 367 | 39.80 | 6.38 | 16 | 18.0% |
46 | P67778 | Prohibitin | PHB | 272 | 29.80 | 5.76 | 2 | 18.0% |
47 | P60335 | Poly(rC)-binding protein 1 | PCBP1 | 356 | 37.50 | 7.09 | 5 | 17.0% |
48 | O55239 | Nicotinamide N-methyltransferase | NNMT | 264 | 29.60 | 5.27 | 3 | 12.0% |
49 | Q9Z0S1 | 3′(2′),5′-bisphosphate nucleotidase 1 | BPNT1 | 308 | 33.20 | 5.54 | 3 | 13.0% |
50 | Q91VJ5 | Pqbp1 protein | PQBP1 | 263 | 30.60 | 5.86 | 3 | 25.0% |
51 | Q9QX98 | ptf1a | PTF1A | 301 | 35.60 | 4.99 | 3 | 9.0% |
52 | Q3TZ89 | Isoform 2 of Protein transport protein Sec31B | SEC31B | 1158 | 125.63 | 8.00 | 2 | 4.0% |
53 | Q01063 | cAMP-specific 3′,5′-cyclic phosphodiesterase 4D | PDE4D | 747 | 84.56 | 4.79 | 4 | 18.0% |
54 | Q61425 | Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial | HADH | 314 | 34.40 | 8.65 | 9 | 21.0% |
55 | P08074 | Carbonyl reductase [NADPH] 2 (Fragment) | CBR2 | 244 | 26.00 | 9.10 | 2 | 5.0% |
56 | E9PWE2 | Transcription factor E2a | TCF3 | 653 | 67.89 | 5.94 | 8 | 49.0% |
57 | Q6ZPW1 | Protein Znf512b | ZNF512B | 883 | 96.58 | 9.80 | 7 | 29.0% |
58 | Q9CPN9 | Protein 2210010C04Rik | 2210010C04Rik | 247 | 27.09 | 8.22 | 2 | 13.0% |
A) Spot number, B) UniProt accession number, C) Number of matched amino-acids, D) Molecular weight, E) Calculated PI (isoelectronic point), F) Number of matched peptides, and G) Coverage percentage.