Table 1.

Bovine viral diarrhoea virus (BVDV) detection and pathology in rabbits at day 5 after challenge by intravenous route (IV) or nebulizer (N)

	BVDV RT-PCR a				Virus isolation	GALT depletion c
Animal	Buffy coat	Ileum	Appendix	Spleen	Ileum
IV5	neg	+	+	+	+	moderate-severe
IV6	+	+	+	+	+	mild-moderate
IV7	+	+	+	+	+	moderate-severe
IV8b	neg	neg	neg	neg	neg	none
N5	neg	+	+	+	not done	indeterminatec
N6	neg	neg	+	+	not done	mild-moderate
N7	neg	neg	neg	+	not done	mild-moderate
N8b	neg	neg	neg	neg	not done	indeterminatec

^aRT-PCR for viral RNA detection was performed as described in the materials and methods. In each case, 1 μL of total RNA was used in assays specific for BVDV type 1 and for genomic β-actin. Assays with Ct for BVDV and for actin less than 40 were considered positive (+), while assays with Ct for BVDV greater than 40.1 and Ct for actin less than 40 were considered negative (neg).

^bnegative control animals.

^cGut associated lymphoid tissue (GALT) depletion was assessed by histopathology as described in the materials and methods section. In group N, GALT depletion could not be determined clearly in two animals due to autolysis.