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. 2014 Jul;112:26–37.

FIGURE 6.

FIGURE 6

CD40 ligand- and TSH-mediated IL-8 transcription is suppressed by Akt inhibitor IV in cultured fibrocytes. In this experiment the role of Akt phosphorylation in IL-8 production was demonstrated at the pretranslational level. Akt is a common member of the TSHR and CD40 signaling pathways, which partake in the onset of thyroid-associated orbitopathy. Cells were stimulated for 6 hours with CD40 ligand or TSH with or without Akt inhibitor (AKTi) added 1 hour prior. CD40 ligand- or TSH-treated cultures significantly increased IL-8 mRNA compared to unstimulated cultures (n=3, **P <.01). Pretreatment for 1 hour with AKTi abrogated CD40 ligand- and TSH-mediated IL-8 mRNA expression (n=3, **P <.01; ***P <.001).