Figure 3. Liver injury depends on the presence of hepatic MDSC with damage-inducing potency.

EL4 tumor-bearing mice were treated with 5-FU or saline. Liver immune cells were analyzed for MDSC and MDSC subsets and mouse serum was analyzed for ALT and AST levels (A) (N = 6 mice per treatment group, 2 independent experiments). B, 5×10⁷ CD11b⁺ cells isolated from livers of indicated untreated subcutaneous tumor-bearing mice were injected intravenously into naïve or RIL175 tumor-bearing recipient mice and ALT and AST serum levels were analyzed 16 h after transfer (N≥6 recipient mice, 2 independent experiments). Data are expressed as mean ±SEM. **p<0.01, ***p<0.001, ****p<0.0001 (A was analyzed by two-tailed Student's t test. B was analyzed by One-way ANOVA).