Table 1. Primers and probes for Taqman real time RT PCR assays targeting the N protein gene of the two salmon louse rhabdoviruses, LSRV-No9 and LSRV-No127.
Code | Sequence | Position |
No9-NF | 5′-TCC AAC AGA TCT CCT TAC TCA GTC A -3′ | 922–946 |
No9-Nprobe | 5′- CGC CAA TCC CTT ATT -3′ | 948–962 |
No9-NR | 3′- TCC AAT GAT ATG GAC CCA CAT G – 5′ | 987–966 |
No127-NF | 5- CTA TGG AGC CAT CGG AGG TTA T -3′ | 873–894 |
No127-Nprobe | 5′- ACC TGG GTG ACT CTT -3′ | 896–910 |
No127-NR | 5′- CAA GAT CTC AGT CGA GAC GGA AT -3′ | 934–912 |
The position of the primer and probes are related to the ORF of the N protein gene of the two viruses.