Table 4.
GO enrichment classification of alternatively spliced genes after hypoxia
| Pathways | Gene symbol | Accession number | Splicing Index | Probe location | Gene description |
|---|---|---|---|---|---|
|
Apoptosis-associated |
|
|
|
|
|
| Induction of apoptosis by intracellular signals |
SART1 |
NM_005146 |
0.46 |
Exon11 |
Squamous cell carcinoma antigen recognized by T cells |
| Positive regulation of neuron apoptosis |
PTPRF |
NM_002840 |
2.56 |
Exon34 |
Protein tyrosine phosphatase, receptor type, F |
| Apoptosis |
PPP1R13L |
NM_006663 |
2.01 |
Exon13 |
Protein phosphatase 1, regulatory (inhibitor) subunit 13 like |
| |
STEAP3 |
NM_182915 |
2.22 |
Exon6 |
STEAP family member 3 |
| TNFRSF14 |
NM_003820 |
2.39 |
Exon8 |
Tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) |
|
| Negative regulation of apoptosis |
ERCC2 |
NM_001130867 |
0.42 |
Exon8 |
Excision repair cross-complementing rodent repair deficiency, complementation group 2 |
| Anti-apoptosis |
SERPINB2 |
NM_002575 |
0.48 |
Exon8 |
Serpin peptidase inhibitor, clade B (ovalbumin), member 2 |
|
Proliferation-associated |
|
|
|
|
|
| Cell growth |
ACTA1 |
NM_001100 |
0.22 |
Exon6 |
Actin, alpha 1, skeletal muscle |
| NDRG4 |
NM_001130487 |
2.62 |
Exon5 |
NDRG family member 4 |
|
| Cell cycle checkpoint |
ERCC2 |
NM_001130867 |
0.42 |
Exon8 |
Excision repair cross-complementing rodent repair deficiency, complementation group 2 |
| Multicellular organism growth |
ERCC2 |
NM_001130867 |
0.42 |
Exon8 |
Excision repair cross-complementing rodent repair deficiency, complementation group 2 |
| Cell proliferation |
ERCC2 |
NM_001130867 |
0.42 |
Exon8 |
Excision repair cross-complementing rodent repair deficiency, complementation group 2 |
| MT3 |
NM_005954 |
2.72 |
Exon3 |
Metallothionein 3 |
|
| Cell cycle arrest |
SART1 |
NM_005146 |
0.46 |
Exon11 |
Squamous cell carcinoma antigen recognized by T cells |
| Positive regulation of cell proliferation |
IL11 |
NM_000641 |
7.99 |
Exon5 |
Interleukin 11 |
| Negative regulation of cell proliferation |
PTPRF |
NM_002840 |
2.56 |
Exon34 |
Protein tyrosine phosphatase, receptor type, F |
|
Migration-associated |
|
|
|
|
|
| Axon guidance |
SLIT3 |
NM_003062 |
0.44 |
Exon1 |
Slit homolog 3 (Drosophila) |
| SEMA5A |
NM_003966 |
2.90 |
Exon23 |
Sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A |
|
| Motor axon guidance |
EGR2 |
NM_000399 |
5.08 |
Exon2 |
Early growth response 2 |
| Cell migration |
PVR |
NM_001135770 |
0.49 |
Exon3 |
Poliovirus receptor |
| Positive regulation of cell migration |
LAMB1 |
NM_002291 |
0.46 |
Exon9 |
Laminin, beta 1 |
| Regulation of cell shape |
ARAP1 |
NM_001135190 |
0.45 |
Exon5 |
ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 |
|
Angiogenesis-associated |
|
|
|
|
|
| Positive regulation of angiogenesis |
RUNX1 |
NM_001122607 |
0.40 |
Exon1 |
Runt-related transcription factor 1 |
| Wound healing | SERPINB2 | NM_002575 | 0.48 | Exon8 | Serpin peptidase inhibitor, clade B (ovalbumin), member 2 |
Differentially spliced genes in human MSCs under hypoxia, were subjected to GO analysis as described in Methods.