Figure 1. rBmHAT specific IgG antibodies in the sera of macaques.

Macaques were immunized three times at four weeks interval with 200 µg of rBmHAT combined with alum (AL007) adjuvant. Sera collected one month after the final immunization was analyzed for IgG titer by ELISA. Approximately, 100 ng of each recombinant protein (100 ng/100 µl/well) were coated onto the wells of an ELISA plate and bound serum IgG was detected using a HRP labeled anti-monkey IgG secondary antibodies. Each bar represents mean ±S.D of 5 animals. Significant **P<0.001 titer of antibodies observed compared to other antigens.