Table 2. Increase in IFN- γ production following rBmHAT antigen stimulation.
Control (alum only) | rBmHAT(Immunized with rBmHAT + alum) | |||||||||
Animal I.D | 4995 | 5240 | 5249 | 5252 | 5253 | 4996 | 5242 | 5254 | 5258 | 5259 |
IFN-γ secretion (pg/ml) | 0 | 0 | 0 | 0 | 0 | 0 | 62.5 | 0 | 62.5 | 62.5 |
PBMC were isolated after the final immunization and subjected to rBmHAT antigen stimulation. Supernatants from PBMC cultures were harvested upon 5 days of stimulation and assayed in ELISA for IFN- production.